A workflow to increase verification rate of chromosomal structural rearrangements using high-throughput next-generation sequencing
نویسندگان
چکیده
منابع مشابه
A workflow to increase verification rate of chromosomal structural rearrangements using high-throughput next-generation sequencing.
Somatic rearrangements, which are commonly found in human cancer genomes, contribute to the progression and maintenance of cancers. Conventionally, the verification of somatic rearrangements comprises many manual steps and Sanger sequencing. This is labor intensive when verifying a large number of rearrangements in a large cohort. To increase the verification throughput, we devised a high-throu...
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Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2014
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114189